| Congresso Brasileiro de Microbiologia 2023 | Resumo: 36-1 | ||||
Resumo:Caries represents a prevalent public health concern, affecting approximately 45.16% of the global population. This oral condition involves intricate interactions, including biofilm formation, and contributes to the development of additional pathological conditions. In the pursuit of antimicrobial agents, metallic compounds have garnered significant attention. In this scenario, copper has emerged as a central metal for medicinal applications due to its essential nature and relatively lower toxicity compared to other metals. Integrating copper with a metallic ion confers advantageous attributes such as a singular mechanism of action, multiple targets, and the ability to avoid the emergence of resistance. These distinctive characteristics pose challenges when one attempts to achieve analogous effects solely through organic structures. This study aimed to evaluate the antibiofilm activity and cytotoxicity of copper(II) associated with thiosemicarbazone. For this purpose, CuCl(atc-Me) (sample I) and [{Cu(µ-atc-Me)}2 µ-SO4 (sample II) were used to carry out the assays of Minimum Inhibitory Concentration of Biofilm (MICB50), determined as the lowest concentration that was able to inhibit biofilm formation by at least 50%, as well as cell viability (Log10 CFU/mL) assays against Enterococcus faecalis (ATCC 4082), Lactobacillus paracasei (ATCC 11578), Streptococcus mutans (ATCC 25175), and S. salivarius (ATCC 25975). In addition, the cytotoxicity to the GM07492A cell line was tested in vitro, and LC50 was calculated. For sample I, the MICB50 values ranged from 0.78 to 6.25 µg/mL. The best results were obtained against L. paracasei (0.78 µg/mL) and S. mutans and S. salivarius (3.12 µg/mL). The cells were eliminated at 50, 1.56, and 6.25 µg/mL sample I, respectively. For sample II, MICB50 was 1.56, 6.25, and 12.5 µg/mL against S. mutans, S. salivarius, and L. paracasei, respectively; cells were eradicated at 6.25 µg/mL sample II for S. salivarius. Samples I and II inhibited cell viability at 2.9±0.9µg/mL and 4.9±0.2 µg/mL, respectively. Therefore, the samples have potential antibiofilm activity against cariogenic bacteria at concentrations below their cytotoxic concentration. Palavras-chave: copper, thiosemicarbazone, oral pathogens, antibiofilm activity, cytotoxicity Agência de fomento:Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |||||